Top-Down SequencingThe use of Travelling wave and drift tube IMS separations for separating protein ion charge states and their fragments
Although extensive work involving the separation and
characterization of protein and peptide ions has been carried out, few
studies have examined the mobilities of fragment ions. In this
study, two new ion mobility spectrometry (IMS) techniques, travelling
wave (TWIMS) and a tandem drift tube (TDTIMS) have been used to
separate the ubiquitin fragment ions (formed by collision induced
dissociation) prior to analysis by MS.