A Comparison of Matrix Effects, Speed and Sensitivity in UPLC and HPLC
ASMS 2006 Micro and Nano Scale Separation: Use mobile phase pH
to move compounds away from interferences and improve retention,
resolution and sensitivity. Mixed-mode solid phase extraction results
in cleaner extracts than PPT, and improves method robustness by
reducing buildup on LC columns. UPLC™ provides increased resolution
from endogenous compounds and closely related compounds over HPLC.
UPLC™ improves S/N over HPLC, which improves LOD/LLOQ, helping to
compensate for ion suppression.The combination of mixed-mode SPE and
UPLC™ with high pH for ionizable bases is the best solution for
minimizing matrix effects in bioanalytical analyses.