Transferring Existing HPLC Methods to UPLC™: A Systematic Approach

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Part Number:
Eric S. Grumbach, Thomas E. Wheat, Jeffrey R. Mazzeo [Waters]
HPLC 2006, June 17-23, San Francisco, CA
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Content Subtype:
Caftaric acid; Chlorgenic acid; Cynarin; Echinacoside; Cichoric acid
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Ultra Performance LC™ is an analytical tool that has resulted from the evolution of column chemistry and instrumentation. This technique can be used to develop new analytical methods, but it also finds utility in improving the speed, sensitivity, and resolution of existing methods.To transfer an existing HPLC method to a highly efficient UPLC method, the properties of the instrument, operating conditions and the column chemistry must be controlled and matched. Geometric considerations and scaling will usually minimize any impact of the instrument and the procedures. The subtleties of column chemistry have a less predictable effect on the transfer. It is necessary to properly characterize the selectivity relationships between UPLC columns and common HPLC materials to best match retention and selectivity. Final adjustment of mobile phase and operating conditions is often required for particular combinations of sample and column in an established method.

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