Rapid In-Vitro Metabolite ID in Drug Discovery by Ultra Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

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Erin Chambers, Diane Diehl, Jeff Mazzeo[Waters] Mary Lame , Amit Kalgutkar [Pfizer],
HPLC 2006, June 17-23, San Francisco, CA
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Buspirone and metabolites; Indomethacin and a neutral amide derivative
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Rapid, early detection and identification of major metabolites has long been held as a crucial role for scientists involved at the discovery stage of drug development. In contrast to the later development phase, discovery scientists are typically flooded with compounds. For each of these potentially diverse chemical entities, early detection and identification of metabolites is important for several reasons: the identification of potentially toxic metabolites, assessment of metabolites as potential active ingredients, and to obtain an understanding of the rate and route of metabolism. With many discovery labs being filled with triple quadrupole MS systems, it was our intent to develop a rapid method for identifying and detecting major metabolites for the scientists operating these instruments. The introduction of Ultra Performance LC (UPLC™) has facilitated this endeavor. The combination of a 1.7 µm packing material and a chromatographic system capable of operating at elevated pressures dramatically increases resolution, speed and sensitivity of typical analyses. In this initial study, we utilize the combination of UPLC™ and triple quadrupole mass spectrometry to identify major metabolites from human liver microsomes. UPLC enables us to dramatically decrease the chromatographic run time, while improving the resolution and sensitivity of the separation.

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