Improvements in Reversed-Phase HPLC Columns Designed for Polar Compound Retention

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Fang Xia, Paula Hong, Bonnie Alden, Cheryl Boissel, Diana Swanson, Erin Chambers, Diane Diehl, Pamela Iraneta, Jeffrey Mazzeo, Kevin Wyndham, Nicole Lawrence, Thomas Walter [Waters]
HPLC 2006, June 17-23, San Francisco, CA
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Water soluble vitamins, Catecholamines,
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Reversed-phase LC columns designed for polar compound retention often have low ligand densities and/or novel ligands in order to maintain pore wetting and enhance retention. Potential weaknesses of these types of columns include shortened lifetimes at low pH (e.g., TFA-containing mobile phase(s)), poor peak shapes above pH 5 and/or MS bleed. To solve these issues, we improved our existing polar retention C18 column by using a trifunctional ligand and new bonding and endcapping processes. We have seen dramatic improvements in peak shapes of basic analytes at pH 7 as well as greatly improved low pH lifetimes (up to a 4-fold improvement). MS response of polar analytes was also measured and compared to other RP polar retention LC columns. Lastly, we show additional applications that are now possible with this new column chemistry.

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