Improved Glycopeptide Fingerprinting and Mass Spectra for Characterization of Recombinant and Isolated Glycoproteins

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Beth L. Gillece-Casto, Thomas E. Wheat, Ziling Lu, and Jeffrey R. Mazzeo
ASMS 2006 May 28 - June 1 Seattle, Wa
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ASMS Glycoproteins II Chromatographic resolution provides excellence in mass spectra of glycopeptides. High resolution separation of glycopeptides has been achieved on 300A and 130A pore size 1.7 ƒÊm particles.Good peak shape is obtained for sialylated and nonsialylated glycopeptides including positional isomers. Changing the gradient slope, temperature or mobile phase modifier can optimize separations. Data obtained in MS only mode produced spectra characterizing complex sialylated glycan structures.

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