Improved Glycopeptide Fingerprinting and Mass Spectra for Characterization of Recombinant and Isolated Glycoproteins
ASMS Glycoproteins II Chromatographic resolution provides
excellence in mass spectra of glycopeptides. High resolution separation
of glycopeptides has been achieved on 300A and 130A pore size 1.7 Êm
particles.Good peak shape is obtained for sialylated and nonsialylated
glycopeptides including positional isomers. Changing the gradient
slope, temperature or mobile phase modifier can optimize separations.
Data obtained in MS only mode produced spectra characterizing complex
sialylated glycan structures.