Improved Glycopeptide Fingerprinting and Mass Spectra for Characterization of Recombinant and Isolated Glycoproteins

Library Number:
Part Number:
Ziling Lu;Beth L. Gillece-Castro;Thomas E. Wheat;Jeffrey R. Mazzeo[Waters]
ASMS 2006 May 28-June 1 Seattle, Wa
Content Type:
Content Subtype:
Bovine fetuin, Mouse IgG,
Tryptic digest
Related Products:
Characterization of glycoproteins entails analysis of the primary structure of the protein and analysis of the attached oligosaccharides. Reversed-phase peptide mapping has come to be the standard for assessing the primary structure. The common glycan mapping techniques are used to establish the pattern of sugar structures associated with the protein but cannot reveal site-specific structures because the sugars are first released from the protein. In peptide maps, the intact glycopeptides are separated so that the structures of the oligosaccharides can be associated with a particular site of attachment. The glycopeptides, however, often give incomplete separations and distorted peaks. With chromatographic techniques based on sub-2µm particles, these structures are characterized more completely.

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