An Automated High Throughput Approach to Quantification of Multiple Compounds Using UPLC Coupled to Tandem Mass Spectrometry
We have demonstrated the feasibility of generating good quality
quantitative data using a highly automated UPLC/ MS/MS methodology from
samples in dirty matrix. From the data available UPLC/MS/MS gave
between 2x and 3x improvement in signal/noise when compared to
HPLC/MS/MS.The UPLC/MS/MS gave 3x the throughput of the HPLC/MS/MS
method while giving highly comparable results.