Streamlining HPLC Method Development

Library Number:
Part Number:
Kevin Jenkins, Diane Diehl, Jeffrey Mazzeo [Waters]
EAS 2005
Content Type:
Content Subtype:
Related Products:
Method developers change the pH of the mobile phase to manipulate the selectivity of the separation. For many ionizable analytes, significant shifts in pH result in large changes in selectivity. Additionally, for basic analytes, using a mobile phase two units above its pKa will ensure that the analyte is not charged, resulting in better retention and reduced tailing. However, chromatographers have avoided using elevated pH mobile phases because traditional silica columns dissolve under these conditions. With the advent of hybrid inorganic/organic stationary phases, running separations at higher pH is now part of many method development approaches. As part of ongoing research into the field of hybrid organic/inorganic particles as HPLC packing materials, a new bridged ethyl hybrid particle has been developed. This second generation technology results in columns that are more efficient and stable at high and low pH and provide improved peak shapes for basic compounds. In this research, we will utilize the extremes of pH to outline method development strategies that take advantage of both the high pH stability and available ligands.

Title Format File Size
wa43196 PDF 925.86kB