Recent Advances in UPLC Column Technology

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Eric S. Grumbach, Thomas E. Wheat and Jeffrey R. Mazzeo [Waters]
Pittcon, March13-16, 2006
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UPLC allows chromatographers to develop higher resolution, faster chromatographic methods by improving system efficiency. This is achieved by employing 1.7 µm particle packed columns and low dispersion, high pressure instrumentation. New advancements in UPLC column technology have been developed to achieve even higher resolution chromatographic methods. Shorter 30 mm columns are utilized for fast method screening. Once a promising set of conditions is observed, this method is scaled to longer length columns to improve resolution. Additionally, temperature is explored as a parameter to manipulate selectivity. In this lecture we discuss these new advancements in UPLC column offerings.

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