Development of an Automated RP/RP 2D Nano LC/MS Method for Proteomic Analysis

Library Number:
720002272EN
Part Number:
720002272EN
Author(s):
James Murphy, Hongji Li, Scott Geromanos, John Gebler
Source:
ASMS 2007, June 1 - 7, 2007, Indianapolis, IN
Content Type:
Posters
Content Subtype:
ASMS
Related Products:
nanoACQUITY UPLC
An automated RP/RP 2D nanoLC/MS method was developed; the orthogonality of the two RP (Reverse Phase) dimensions was realized by running the separations at sharply different pH. The automation of the system was made possible by using a novel online dilution method: the organic mobile phase eluting from the first dimension was diluted with an aqueous mobile phase before being collected for separation on the second dimension. More efficient fractionation was observed with RP than with SCX. Highly reproducible protein identification results were obtained with the system.

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