Shorter, Faster, Smarter: Development of New 4.6 x 20 mm Column Hardware for Fast Separations

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Diane Diehl, Eric Grumbach, Jon Belanger, Michael Savaria, Kim Tran, Uwe Neue, Jeff Mazzeo
CO WW Meeting 2002
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Other Symposium
XTerra RP18 5 µm Steel 4.6 mm 20mm XTerra MSC18 5 µm Steel 4.6 mm 20 mm
Pharmaceutical companies have been driven to cut costs by increasing sample throughput. Because of the mandate to run faster separations, researchers have tried to move away from hour long separations on 15 or 25 cm columns. Many have turned to the monolith technology due to claims of dramatic reductions in separation time, reduction of column back pressure to minimum, and significantly increased sample throughput. However, running at such high flow rates (4 to 9 mL/min) increases solvent consumption dramatically. Additionally, monolith technology is not yet available in preparative dimensions which create a barrier to direct scale-up possibilities. New column hardware in 4.6 x 20 mm dimensions has been developed and packed with 2.5 µm and 3.5 µm XTerra® MS C18 particles. We have measured the peak capacities and back pressures of these columns and found them to be comparable to results obtained with the monolith columns. We have now developed several gradient and isocratic applications with total run times of 5 minutes or less, using reasonable flow rates. We will show how an application run on a long column can be transferred to these shorter columns resulting in optimum chromatographic performance in a much shorter run time.