Improved SPE for LC-MS Determination of Ractopamine in Porcine and Bovine Liver: The Oasis MCX Method Using Otto SPEcialist

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Nathan Stebbins, Jessica Lance, Joe Bruton, Jeremy Shia, Marina Obradovic, Kathryn Brennan, Claire Rhodes
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This application brief describes a simple, rapid, and effective cleanup strategy to remove co-extractives from porcine and bovine liver extracts. This is executed via Oasis MCX 96-well SPE plate processed using Otto SPEcialist, a semi-automated positive pressure manifold prior to UPLC-MS/MS quantification of total ractopamine, a beta-agonist veterinary drug, with a limit of detection of 0.1 ng/g. This method quantifies ractopamine and ractopamine-glucuronide metabolites to accurately measure ractopamine in animal tissue. The use of Otto SPEcialist to process samples in 96-well plate format not only increases sample throughput and reproducibility, but also eliminates the risk of cross contamination when using manual vacuum manifold.

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