Analysis of siRNA Duplexes at Non-Denaturing UPLC Conditions Using MaxPeak Premier Column Technology

Library Number:
APNT135088510
Part Number:
720007361EN
Author(s):
Pratheeba Yogendrarajah, Willy Verluyten, Evelien Dejaegere, Leslie Napoletano, Jean-Paul Boon, Mario Hellings , Martin Gilar
Source:
Analytical Development, Janssen Pharmaceutical Companies of Johnson & Johnson, Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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Ion-pair reversed-phase non-denaturing IP-RP LC method is suitable for separation of single stranded impurities from siRNA duplex. The optimized ion pairing system permitted separation and characterization of two siRNA duplexes present in the drug formulation. Mobile phase was compatible with MS detection which permitted the confirmation of eluting species according to their molecular weight. We demonstrate that ACQUITY Premier Peptide BEH C18, 300 Å Column is suitable for robust quantitation of siRNA duplexes without compromises or carryover. The ACQUITY Premier Column afforded higher confidence in accuracy of quantitation; its performance fulfils the quality requirements for the qualification parameters of recovery, repeatability, and linearity.


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