Rapid Analysis of Synthetic mRNA Cap Structure Using Ion-Pairing RPLC with the BioAccord LC-MS System

Library Number:
APNT135087359
Part Number:
720007329EN
Author(s):
Jennifer M. Nguyen, Siu-Hong Chan, Bijoyita Roy, Martin Gilar, Brett Robb, Weibin Chen, Matthew A. Lauber
Source:
Waters, New England Biolabs Inc.
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
Biopharmaceutical
 
 
 
 

This application note demonstrate that a rapid LC-MS method applicable to evaluating the extent of 5’ capping of synthetic mRNA, an important CQA for synthetic mRNAs, using an ACQUITY Premier Oligonucleotide BEH C18 Column couple with a BioAccord LC-MS System. The ACQUITY Premier Column with MaxPeak High Performance Surfaces Technology could provide considerable improvements in RNA recovery upon first injection. Paired with the BioAccord LC-MS, this combination of technologies allows for accurate quantitation, even at low limits of detection, such that it is possible to validate the manufacturing of Cap-1 mRNA molecules, and potential presence of product precursor related impurities.


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