Improving Recovery and Quantitation of Oligonucleotide Impurities Using MaxPeak HPS Technology

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Brooke M. Koshel, Robert E. Birdsall, Ying Qing Yu
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Oligonucleotides are notoriously difficult to characterize and quantify due to non-specific adsorption to metal surfaces, which is especially true of trace-level species and low sample loads. This work demonstrated that the ACQUITY Premier Solution could be used to enhance recovery for more reliable quantitation of impurities compared to a more traditional analysis using a stainless-steel column and an LC system containing a metal flow path. The best performance was achieved through the combination of an ACQUITY Premier Column and ACQUITY Premier System, which showed greater recovery, lower detection limits, and increased linearity and reproducibility.

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