The method validation study results demonstrate a robust analytical method for the determination of cationic polar pesticides using the established QuPPe extraction protocol with analysis using an ACQUITY UPLC BEH Amide Column fitted in an ACQUITY UPLC I-Class PLUS System coupled to a Xevo TQ-S micro. The results for most compounds exceed the requirements for method validation in the SANTE guidelines. The trueness and precision of this LC-MS/MS method determined at two matrix QC levels with 5 replicate injections was found to be acceptable for all compounds. Retention time stability and robustness was proven over the course of the study with RSDs for all compounds under 2% across all method performance batches. In most cases, limits of quantification surpass the MRL requirements. The short method run time of 10 minutes and utilizing the QuPPe extraction method allows a high sample throughput for the analysis of the cationic polar pesticides in various food commodities investigated.