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Determination of Cationic Polar Pesticides and Plant Growth Regulators Using UPLC-MS/MS with the ACQUITY UPLC BEH Amide Column
The method validation study results demonstrate a robust analytical method for the determination of cationic polar pesticides using the established QuPPe extraction protocol with analysis using an ACQUITY UPLC BEH Amide Column fitted in an ACQUITY UPLC I-Class PLUS System coupled to a Xevo TQ-S micro. The results for most compounds exceed the requirements for method validation in the SANTE guidelines. The trueness and precision of this LC-MS/MS method determined at two matrix QC levels with 5 replicate injections was found to be acceptable for all compounds. Retention time stability and robustness was proven over the course of the study with RSDs for all compounds under 2% across all method performance batches. In most cases, limits of quantification surpass the MRL requirements. The short method run time of 10 minutes and utilizing the QuPPe extraction method allows a high sample throughput for the analysis of the cationic polar pesticides in various food commodities investigated.
| Title | Format | File Size |
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| Download PDF | 1163.95kB |