Improved Peak Recovery and Peak Shape of Oligonucleotides Using Waters PREMIER Columns

Library Number:
APNT135082201
Part Number:
720007200EN
Author(s):
Kenneth D. Berthelette, Steve Shiner, Matthew A. Lauber
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
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The Waters PREMIER brand of columns reduces the adsorptive interactions between analytes and column hardware surfaces. While problematic secondary interactions can occur across a wide range of applications, separation of oligonucleotides is an important and ever relevant area of separation science. Oligonucleotides are generally very sensitive to metallic surfaces as a result of their structure which can lead to significant challenges when it comes to recovery (peak area) and achieving good peak shape. In this work, an XBridge PREMIER Oligonucleotide BEH C18 Column was compared to a competitor column based on conventional stainless-steel hardware. The PREMIER column yielded higher peak areas and sharper peaks compared to the competitor column. Switching to PREMIER columns will provide better separations and more accurate results. Even more benefits can be found by using PREMIER columns on a PREMIER LC system.


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