Maximizing Phosphopeptide Recovery in LC-MS Studies with MaxPeak High Performance Surfaces Technology

Library Number:
APNT135080180
Part Number:
720007025EN
Author(s):
Chris Hughes, Lee A. Gethings, Robert S. Plumb
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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Treatment of the LC fluidic path with a hybrid organic/inorganic barrier based on an ethylene-bridged siloxane structure have been shown to dramatically improve, in terms of mass spectrometry response and chromatographic peak shape, the recovery response of phosphopeptides in LC-MS studies. This treated surface, known as MaxPeak High Performance Surface (HPS), limits the metal ion interaction with the phosphate group present in the peptide which is the cause of problems seen with phosphoproteomics analyses utilizing a normal LC system. Various peptide containing samples have been studied, with varying degrees of improvement. In the most extreme cases, we observe that certain peptides analyzed in this study go from not being detected with a standard system to very strong signals with an HPS fluidic path.


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