Targeted UPLC-MS/MS Analysis of Oxylipins

Library Number:
APNT135075761
Part Number:
720007030EN
Author(s):
Billy J. Molloy
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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In this application note we present a targeted UPLC-MS/MS methodology for the semi-quantitative profiling of bioactive oxylipins (oxidized fatty acids) in plasma/serum and cell culture media. The combination of mixed mode solid-phase extraction (Oasis MAX µElution SPE) and UPLC-MS/MS provides a broad ranging analysis of oxylipins for use in targeted workflows. Retention times and MRM transitions for 92 oxylipins are detailed for routine, targeted analysis of samples in biomedical research studies.


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