Developing a Quantitative Surrogate Peptide Assay – Peptide mapping through MRM Optimization for Measuring Dulaglutide in a Rat PK Study
The work described here includes a complete workflow for the identification and optimization of surrogate peptides. This workflow has been applied to the development of a dulaglutide quantitative assay. Combined with advanced sample preparation techniques, rat PK samples were analyzed with high accuracy and precision.
- Peptide mapping experiments were used to identify and refine a list of surrogate peptides to be pursued for the quantification of dulaglutide
- MassLynx Skyline Interface software was implemented to identify and optimize MRM transitions for chosen surrogate peptides
- Dulaglutide was quantified in rat PK samples with high precision and accuracy over the range of 0.15–98.21 nM