GlycoWorks RapiFluor-MS Automation Using the Andrew+ Pipetting Robot

Library Number:
APNT135072949
Part Number:
720006971EN
Author(s):
Corey E. Reed, Stephan M. Koza, Steven Calciano
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:
 
 
 
 

Using the Andrew+ pipetting robot from Andrew Alliance - A Waters Company, the GlycoWorks RapiFluor-MS N-Glycan Labeling Kit was automated and verified for performance. Verification requirements included comparability to manually performed preparations of the same sample number with ≤25% deviation in total area and ≤5% deviation in relative area. During verification, it was evident that protocol optimization was required due to changes in consumables causing non-specific sample losses of 45% on average during the automated protocol. Changes were made to mitigate this sample loss, and ultimately the automated protocol showed 88% recovery compared to the manual protocol. Throughout the optimization process relative areas remained highly reproducible, and the final protocol showed no relative area deviation >2.6% from the manual procedure.


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