GlycoWorks RapiFluor-MS Automation Using the Andrew+ Pipetting Robot

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Corey E. Reed, Stephan M. Koza, Steven Calciano
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Using the Andrew+ pipetting robot from Andrew Alliance - A Waters Company, the GlycoWorks RapiFluor-MS N-Glycan Labeling Kit was automated and verified for performance. Verification requirements included comparability to manually performed preparations of the same sample number with ≤25% deviation in total area and ≤5% deviation in relative area. During verification, it was evident that protocol optimization was required due to changes in consumables causing non-specific sample losses of 45% on average during the automated protocol. Changes were made to mitigate this sample loss, and ultimately the automated protocol showed 88% recovery compared to the manual protocol. Throughout the optimization process relative areas remained highly reproducible, and the final protocol showed no relative area deviation >2.6% from the manual procedure.

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