Size Exclusion Chromatography Method Transfer from a Standard HPLC to an Arc HPLC System

Library Number:
APNT135071867
Part Number:
720006962EN
Author(s):
Zhimin Li, Paula Hong, and Patricia R. McConville
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 

This application brief demonstrates a size-exclusion chromatography (SEC) method transfer for monoclonal antibody (mAb) analysis from an industry-standard HPLC system to a Waters Arc HPLC System. Comparable retention time, percent area of monomer, high molecular weight species, and low molecular degradants and repeatability were obtained from both systems.

Benefits

  • Seamless SEC method transfer across a standard HPLC system and Arc HPLC System
  • Arc HPLC System provides tight RSD for SEC analysis for size variants of a monoclonal antibody (mAb)

Title Format File Size
Download PDF PDF 609.01kB