How to Maximize Bioanalytical Performance of Fc-Fusion Proteins: Practical Sample Preparation and LC-MS/MS Workflows for Dulaglutide Quantification from Plasma

Library Number:
APNT135053877
Part Number:
720006823EN
Author(s):
Caitlin M. Dunning, Mark D. Wrona
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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This application note describes a complete sample preparation workflow for quantitative assay development of Fc containing therapeutics. With an Fc specific immunoaffinity capture method, fast and reproducible digestion workflow, and selective SPE, lower limits of quantification of 1 ng/mL using 50 μL of plasma were achieved.


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