Analysis of Glyphosate, AMPA, and Glufosinate in Water Using UPLC-MS/MS

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Gerdien van Genderen-de Kloe, Bas Muilwijk, Wil van Spaandonk, Marijn Van Hulle, and Simon Hird
Aqualab Zuid and Waters
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Application Notes
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Application Notes
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Numerous laboratory and field studies have been performed to investigate the transport of glyphosate and/or AMPA to the aquatic environment indicating some recognition and concern that these substances can move towards surface waters. 

The difficulties associated with determination of these compounds at trace levels in water samples are related to their high solubility in water, ionic nature, and chelation with metal ions. All three compounds can be derivatized to less polar compounds for improved retention and separation using solid phase extraction (SPE) and reversed-phase liquid chromatography (LC). Fluorenylmethyloxycarbonyl (FMOC) chloride is the most common pre-column derivatization reagent used for this analysis, and it can be successfully used in combination with LC-MS/MS for the determination of all three compounds in one method as part of water monitoring programs.

This application note describes a method for the determination of glyphosate, AMPA, and glufosinate in water samples, without SPE, after derivatization with FMOC, by LC-MS/MS using the ACQUITY UPLC I-Class System coupled to the Xevo TQ-XS using a novel ionization technology, UniSpray.

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