Using High Speed/High Resolution Size Exclusion Chromatography Separation of Polymeric Materials with Dynamic and Multi-Angle Light Scattering Detection

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Jennifer Gough, Robert Birdsall, Damian Morrison, John Champagne , and Kyle Rogers
Waters and Wyatt
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Polymeric materials are an integral part of everyday tasks, and there is great complexity behind the research and manufacturing of these high quality and competitive products. From the simple task of carrying food in biodegradable shopping bags to battling diseases with intricate drug delivery systems, these everyday tasks are possible through an exacting polymer process. As technologies advance, there is a drive to understand and predict material properties through more expansive characterization and flexible analytical instrumentation. Creative analytical solutions, ranging from high speed chromatographic separation to advanced mass spectrometry, have become the  answer for complex product knowledge.

Traditional analytical solutions have often been the answer for polymer characterization. Coupling liquid chromatography (LC) with detectors such as a refractive index (RI), ultra violet or photo diode array (UV or PDA), evaporative light scattering (ELS) and fluorescence (FLR) have met traditional needs. Examples of creative analytical solutions include adding a secondary LC system for two dimensional (2D) chromatography or hyphenating the LC system with detectors such as dynamic light scattering (DLS) and
multi-angle light scattering (MALS).  

 LC separations with resolution and speed have not always been synonymous. Yet, with the innovative design of Waters Advanced Polymer Chromatography (APC) System, including the robust separation column and low dispersion flow path, the system yields high resolution, short analysis times, and quick mobile phase changes. This high speed/high resolution separation technique works best with low dispersion detectors, availability and diversity of which continue to increase as the advantages of APC become more widely used in research and industry.

 In this poster note, Waters APC System is matched well to the experimental conditions available with Wyatt's μDAWN, enabling greater characterization for the complex properties of polymers and proteins.

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