Standard methods for isoflavones in dietary supplements have been established by organizations such as USP and AOAC. These methods use reversed-phase LC with C18 columns and ultraviolet and visible light (UV-Vis) spectroscopy for separation and quantitation. Because of the close structural similarity of these compounds, the chromatographic run times of these methods are typically over 70 minutes long. It is highly desirable to develop a more rapid isoflavone analysis method.
This application note (Part 1 of 3) demonstrates the transfer of the USP method onto an ACQUITY Arc UHPLC System. The analysis time with the ACQUITY Arc System is only 18 minutes, including column wash and equilibration. The ACQUITY QDa Mass Detector was used to expedite the method transfer described in this study. Benefits of this method include:
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