Tobacco specific nitrosamines (TSNAs) are a group of carcinogenic compounds found in tobacco and tobacco smoke. Several priority toxicants including TSNAs have been identified in tobacco and smoke emissions that need to be accurately measured and reported to regulatory bodies. Four different TSNAs are monitored in tobacco product analysis: N-nitrosonornicotine (NNN), 4-(N-methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosoanatabine (NAT), and N-nitrosoanabasine (NAB).
In this technology brief, we describe an optimized UPLC-MS/MS method for TSNA analysis in tobacco products that has been developed using RADAR Technology. The Xevo TQD Triple Quadrupole Mass Spectrometry System employing RADAR functionality provides an innovative platform to monitor both MRM and full scan MS data simultaneously without compromising data quality. The full scan data helps characterize the potential matrix interferences and enables informed decision making during the method development process.