Evaluation of Alternative Ion-pairing Reagents in the Analysis of Oligonucleotides with the ACQUITY QDa Detector

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Robert E. Birdsall and Ying Qing Yu
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Application Notes
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This application note evaluates the applicability of alternative IP reagents in the analysis of oligonucleotides.

As new therapeutic oligonucleotides come to market with novel physicochemical properties, IP-RPLC/MS-based techniques that incorporate alternative IP buffers to TEA:HFIP may be required to achieve optimal chromatography conditions. The Waters OST columns exhibit a high degree of ruggedness when using alternative methods that require high pH and elevated temperatures over extended time periods, and can be incorporated into existing ACQUITY QDa workflows. Collectively, this work demonstrates that Waters offers a cost-effective solution to the challenges facing today’s analysts in oligonucleotide analyses that is flexible, robust, and readily deployable.

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