Unwanted micro-organisms such as bacteria, viruses, and molds can grow wherever there is a source of nutrition and moisture. This unwanted growth may negatively impact human health, interfere with manufacturing processes, damage building structures and spoil consumer goods. The principal defense against deleterious micro-organisms is biocides, commonly classified as disinfectants, preservatives, antifouling products and pest controls. Biocides are additives in household and industrial products.
To protect the environment and human health, many countries regulate biocide use. In the European Union, this is done through the Biocidal Products Directive. In the United States, regulatory control of biocides falls under the EPA and the biocides applications in food and personal health care are regulated by the FDA. Regulations impact the registration, labeling and composition of biocides. Reliable and rapid methods are therefore essential to ensure effective product quality control.
This application note describes a three-minute separation of six biocides using the Waters ACQUITY UPLC PDA System with Empower 3 Software. With PDA library matching and the built-in advanced mathematical algorithms, each biocide in the mixture can be identified and quantified; the analysis is fast and reproducible. The ability to quickly and unambiguously analyze biocide content can facilitate workflow related to the quality control and regulatory needs of biocide containing products. This methodology benefits new product development, product troubleshooting and biocide production.