USP Analysis of Glimepiride on an Alliance HPLC System: Modernization of a USP Method

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Aaron D. Phoebe, Paula Hong, and Patricia R. McConville
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Application Notes
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In this application, the assay for glimepiride was performed according to the USP monograph.

Using the ACQUITY Columns Calculator, the USP assay for glimepiride was scaled to a column with a smaller particle size and a shorter length column. Additional parameters, including flow rate and injection volume, were also scaled accordingly. These modifications allowed for a reduction in the run time of over 50% and a significant reduction in the mobile phase consumed, while still meeting system suitability criteria – thereby illustrating the potential for method modernization on traditional HPLC systems.

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