USP Analysis of Glimepiride on an Alliance HPLC System: Modernization of a USP Method

Library Number:
APNT134892351
Part Number:
720005695EN
Author(s):
Aaron D. Phoebe, Paula Hong, and Patricia R. McConville
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 

In this application, the assay for glimepiride was performed according to the USP monograph.

Using the ACQUITY Columns Calculator, the USP assay for glimepiride was scaled to a column with a smaller particle size and a shorter length column. Additional parameters, including flow rate and injection volume, were also scaled accordingly. These modifications allowed for a reduction in the run time of over 50% and a significant reduction in the mobile phase consumed, while still meeting system suitability criteria – thereby illustrating the potential for method modernization on traditional HPLC systems.


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