Improving Detection of Anabolic Steroids in Sports: Simultaneous Detection of Intact Phase I and Phase II Urinary Metabolites by UPLC-MS/MS

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G. Balcells, A. Esquivel, O.J. Pozo, J. Segura, R. Ventura
Waters, Hospital del Mar Medical Research Institute, Universitat Pompeu Fabra
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Application Notes
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Application Notes
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This application note presents a novel LC-MS/MS screening method for the simultaneous detection of intact phase I and phase II (glucuronides and sulfates) urinary metabolites of anabolic androgenic steroids (AAS).

A UPLC-MS/MS screening method for the simultaneous detection of steroid metabolites is a feasible alternative compared to the conventional procedures. Incorporation of new phase II metabolites as described is straightforward and can significantly enhance the screening and detection capabilities of steroids in sport.

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