Performance Comparison of a Scaled USP Method for Fluconazole Using ACQUITY UPLC and Competitive UHPLC Instrumentation

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Jennifer Simeone, Paula Hong and Patricia R. McConville
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Application Notes
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Application Notes
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In this application note the USP monograph for fluconazole and related impurities will be scaled from the original HPLC method to a UPLC method and will be run on multiple instruments to show the differences in performance due to individual instrument characteristics.

The ACQUITY UPLC Columns Calculator is a useful tool when scaling methods by providing the user with scaled flow rates, injection volumes, and gradient tables when applicable. In this example for the analysis of fluconazole and fluconazole related compounds A, B, and C, the run time was decreased by more than 65%, and solvent usage was decreased by more than 85%. Additionally, the ACQUITY UPLC I-Class system provides superior chromatographic performance (due to the lower volume of dispersion over that of UHPLC systems) with increased resolution, increased theoretical plates and more symmetrical peak shape.

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