Simple, Standardized, and Sensitive Quantification of Bevacizumab (Avastin) Using ProteinWorks eXpress Digest Kits

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Mary Lame, Hua Yang, Sherri Naughton, and Erin Chambers
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Application Notes
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Application Notes
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Protein Quantification

This application note describes the fast, sensitive quantification of bevacizumab from rat plasma using the ProteinWorks eXpress Digest Kit and Protocol.

The universal, kit-based approach allows novice users to achieve low detection limits with a simple step-wise protocol and a set of standardized, pre-measured reagents, ensuring both the sensitivity required and the transferability desired of such methods.

The kit is optimized and flexible enough to enable simultaneous, sensitive quantification of both unique and generic signature peptides from monoclonal antibodies. This is important as confirmatory data from multiple peptides is critical in supporting the confident use of LC-MS for protein bioanalysis workflows.

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