Effects of Aqueous Sample Content and Aqueous Co-Solvent Composition on UPC2 Separation Performance

Library Number:
APNT134864458
Part Number:
720005505EN
Author(s):
Paul D Rainville, Robert S Plumb, Christopher Knappy, Norman W Smith, Arundhuti Sen
Source:
Waters, King’s College
Content Type:
Application Notes
Content Subtype:
Application Notes
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Here we demonstrate the application of UPC2 for the analysis of polar analytes in aqueous matrices.

UltraPerformance Convergence Chromatography™ (UPC2R) can be used for the direct analysis of polar metabolites from aqueous biological samples, such as urine, or from extracted serum samples. Analytical methods can be rapidly developed from a simple set of starting conditions of column flow-rate, temperature, and pressure. Modifying the co-solvent by the addition of small amounts of water buffer and acid provides rapid high resolution analysis with rapid re-equilibration times.


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