Strategies for Improving Impurity Isolation Using Large Volume Loading and Easy Method Development in Preparative Chromatography

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Jo-Ann M. Jablonski, Andrew J. Aubin, Wendy Harrop
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The isolation and purification of impurities associated with the production of a variety of consumer products must be properly identified and characterized before these commodities can be sold to customers. Using large volume injections, focused gradients, and a change in column selectivity, we have shown that it is possible to effectively isolate a contaminant with excellent purity. This straightforward approach to impurity isolation is easy to adopt for many types of applications.

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