A Streamlined Data Dependent Acquisition (DDA) Peptide Mapping Workflow for Characterizing Therapeutic Proteins Using the Biopharmaceutical Platform Solution with UNIFI

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Liuxi Chen and Ying Qing Yu
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The results presented here demonstrate the dedicated DDA peptide mapping workflow within the Biopharmaceutical Platform Solution with UNIFI. The workflow allows seamless integration of data acquisition, processing, and reporting. Combining the FastDDA acquisition method and the high MS and MS/MS sensitivity provided by the Xevo G2-XS QTof, the UPLC/DDA workflow successfully yields high sequence coverage of the mAbs, and confirms the low level spiked-in peptide at 0.1% level and identifies low level modifications like deamidation and oxidation.

The ability to integrate and automate the DDA peptide mapping workflow greatly reduces the time between data collection and drug development decision-making, subsequently improving the efficiency of laboratories that are challenged with interpreting complex peptide mapping results.

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