Characterization of Biotherapeutics: ACQUITY UPLC H-Class Bio with 2D Part 3 of 3: On-line Enrichment of Low Abundance Species

Library Number:
APNT134836863
Part Number:
720005327EN
Author(s):
Robert Birdsall, Vera Ivleva, Sean M. McCarthy, and Weibin Chen
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
 
 
 

The objective of this application note is to demonstrate that the ACQUITY UPLC H-Class Bio System with 2D Technology is well suited for fractionation and enrichment of low abundant species in biological samples. A therapeutic monoclonal antibody, infliximab, was used to demonstrate this.

This comprehensive three-part series demonstrates that the ACQUITY H-Class Bio System with 2D Technology is well suited for the automated fractionation, desalting, and enrichment of biologically complex samples. The ACQUITY UPLC H-Class Bio System with 2D Technology offers an efficient solution with the flexibility needed for increasing informational content in the characterization of biotherapeutics without compromising productivity.

In this third part of the series, we address how to used 2D UPLC with downstream analyses such as peptide mapping, where peaks of interest may be present in low abundance, and how to apply an enrichment process to ensure digest efficiency and success with the subsequent assay.

Part 1: Online Desalting

Part 2: Direct Interface for IEX with ESI-MS


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