DESI imaging represents a significant enhancement in the capabilities of mass spectrometers to analyze and determine spatial localization and molecular distribution of target molecules within a variety of samples. Use of DESI imaging has the advantages of requiring minimal sample preparation to collect a wealth of molecular information. When optimized, the technique allows for either multiple analyses of a single sample (with different MS polarities if desired) or enables additional visualization techniques (i.e. staining) to be performed on the sample after DESI imaging is complete. As seen is these examples, DESI imaging has been shown to be very effective in analyzing small molecules such as lipids or other small molecule cellular metabolites.