Oral fluid is a highly valuable biological specimen and is commonly used in numerous settings, including therapeutic drug monitoring and testing. Oral fluid analysis has many advantages compared with other matrices such as blood or urine, such as collection convenience and reduced sample collection overheads.
The popularity of the specimen has led to the development of a large variety of collection devices, aiming to further simplify and standardize collection. Typically these devices will include additives and preservatives to improve the stability of the collected sample. One of the key analytical challenges with oral fluid analysis is the limited amount of sample available for testing compared with blood or urine, requiring very sensitive instrumentation to reach the low levels of detection and quantification. The ionKey/MS System offers the capability of improving sensitivity in sample-limited situations, making it ideally suited for this application.
In this technology brief, we demonstrate the separation and high sensitivity detection of opioids in oral fluid with the ionKey/MS System comprised of an ACQUITY UPLC M-Class System in combination with a Xevo TQ-S Mass Spectrometer.