This technology brief demonstrate the increased performance of the SYNAPT™ XS HDMS compared to the SYNAPT G2-Si in terms of sensitivity, resolution, and labile ion transmission for metabolic profiling.
The SYNAPT XS has been shown to provide high quality data for metabolic profiling of urine samples in biomedical research. Thus, depending on the acquisition mode, the mass resolution of representative singly charged compounds is demonstrated to be between 20,000 and 40,000 at FWHM. In addition to the increased resolution, an increase in analytical sensitivity between a factor of two and 18 was also observed, when comparing SYNAPT G2-Si with SYNAPT XS for sensitivity and resolution modes. Implementation of the StepWave XS has shown that the transmission of labile ions is also improved by a factor of 1.2 to 13, with improved precursor to fragment ion ratio. For the spiked components in urine, a typical dynamic range of 3 was also observed.