Improved Analytical Sensitivity and Chromatographic Peak Shape for the Quantification of TCA Cycle Analytes in Human Plasma using the ACQUITY PREMIER System Solution

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Kerri M. Smith, Paul D. Rainville
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Application Notes
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Application Notes
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Targeted Metabolomics

In this application note, we have demonstrated that the analysis of the TCA cycle and related metabolites in human plasma can be achieved with great analytical sensitivity when incorporating MaxPeak HPS Technology into the liquid chromatograph as well as the analytical column. The ACQUITY PREMIER System Solution mitigates analyte interactions with metal to improve peak shape as well as analytical sensitivity without full system passivation with strong acids or chelating additives in the mobile phase. The simple mixed-mode anion exchange separation using the ACQUITY PREMIER CSH Phenyl-Hexyl Column allows for fast separations and easy adaptation.

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