Comprehending COVID-19: Maximizing LC-MS Detection Dynamic Range for Multiple Reaction Monitoring Based SARS-CoV-2 Analysis

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Laurence Van Oudenhove, Jan Claereboudt, Rowan Moore, Hans Vissers, Bart Van Puyvelde, Simon Daled, Dieter Deforce, Katleen Van Uytfanghe, Steve Silvester, Sally Hannam, Donald Jones, Dan Lane, Pankaj Gupta, Leong Ng, Maarten Dhaenens
Waters, University of Ghent, Alderley Analytical, University of Leicester
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Targeted MS-based methods show promise to specifically detect SARS-CoV-2 proteins from various types of biological matrices of COVID-19 patients. Here, the combination of selected chemistries and consumables, using the ACQUITY UPLC I-Class PLUS System and Xevo TQ-XS Tandem Quadrupole Mass Spectrometer, are demonstrated for the detection and quantification of SARS-CoV-2 proteins in nasopharyngeal swabs preserved in Universal Transport Medium. A linear response was achieved across five amount levels for recombinant Spike glycoprotein and Nucleoprotein, using at least two transitions per peptide, whilst maintaining good measurement reproducibility across the detection dynamic range.

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