Quantitative Analysis of THC and its Metabolites in Whole Blood Using LC-MS/MS for Toxicology and Forensic Laboratories

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Xin Zhang, Jonathan P. Danaceau, and Erin E. Chambers
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Application Notes
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Application Notes
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Forensic Toxicology

This application note details the extraction of THC and its metabolites from whole blood samples using a novel SPE sorbent, Oasis PRiME HLB, in a μElution format for forensic toxicology applications.

The unique nature of this sorbent enabled the elimination of conditioning and equilibration steps, simplifying the extraction procedure and speeding up the sample preparation workflow. In addition, the μElution format enabled the direct injection of extracts without evaporation or reconstitution, minimizing the risk of nonspecific binding. One key attribute of this sorbent is its ability to retain phospholipids.

In conclusion, Oasis PRiME HLB has been successfully used to achieve consistent recoveries with minimal matrix effects as well as accurate quantification over 4 orders of magnitude from whole blood samples.

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