Analysis of 25-Hydroxyvitamin D in Serum for Clinical Research

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Robert Wardle, Billy Molloy, and Lisa Calton
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Application Notes
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Application Notes
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This application note describes a clinical research method utilizing Waters® Oasis HLB μElution Plate technology for the extraction of 25OHD2 and 25OHD3 from human serum.

The described semi-automated method overcomes many limitations of current LC-MS/MS method. In particular, several time-consuming and labor-intensive manual sample pretreatment steps have been eliminated. This will enable a wider range of laboratories to implement UPLC-MS/MS methodology for 25OHD analysis in clinical research.

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