Selective Quantitative Determination of Water Soluble Vitamins in Various Food Matrices Using the ACQUITY UPLC H-Class System and the ACQUITY QDa Detector

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Mark E. Benvenuti, Dimple Shah, and Jennifer A. Burgess
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Application Notes
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Application Notes
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Rapid, reliable, and cost-effective methods are required by food manufacturers and ingredient suppliers in order to verify product consistency and ensure that label claims are met. This can be a challenging task with the combination of complex matrices and low fortification levels of some vitamins. In addition, many of the methods currently employed stipulate that the vitamins are either analyzed separately, or in small groups. Established techniques include microbiological assays, colorimetric and fluorimetric analysis, titrimetric procedures and HPLC methodologies. LC-MS offers the opportunity to consolidate methods along with the ability to improve detector selectivity and reduce limits of quantification. In order to offer laboratories the opportunity to capture the benefits of mass detection without the challenges associated with the adoption of mass spectrometers, recent advances in technology have focused on improving instrument usability and robustness. These motivations have resulted in the introduction of the ACQUITY QDa Detector. In this application note, 12 water soluble vitamins (WSVs) were analyzed in dietary supplements and beverage samples using the ACQUITY UPLC H-Class System with t he ACQUITY QDa Detector.

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