High Definition Multiple Reaction Monitoring: The Application of Quadrupole Ion Mobility Time-of-Flight Mass Spectrometry for Targeted Proteomics Studies

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Dominic Helm, Christopher J. Hughes, James I. Langridge, Keith Richardson, Arkadiusz Grzyb, Jason Wildgoose, Bernhard Kuster
Waters, Technische Universitat Munchen
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Application Notes
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Application Notes
Related Products:
Protein Quantification
Protein Quantification
Protein Quantification

The specificity of SRM/MRM peptide quantification has been questioned recently, prompting the use of high-resolution accurate mass instruments for peptide quantification. Here, the benefits of the SYNAPT G2-Si System are demonstrated for the quantification of proteins and peptides in a new High Definition® MRM (HD-MRM) mode, where T-Wave™ ion mobility separations are used with MS/MS mode on a quadrupole time-of-flight mass spectrometer, to provide a highly sensitive and selective quantitative assay with maximum response for all product ions of a molecular peptide ion in a single acquisition.

  • The HD-MRM workflow provides low limits of detection and quantification for all products of a target compound/peptide from a single MS/MS acquisition.
  • HD-MRM acquisitions also enable quantification of specific product ions following acquisition rather than defining the specific target ions prior to acquisition.


***nanoACQUITY UPLC applications readily transfer to the ACQUITY UPLC M-Class System***

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