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Targeted Lipidomics of Oxylipins (Oxygenated Fatty Acids)

Library Number:
APNT134732486
Part Number:
720004664en
Author(s):
Katrin Strassburg, Billy Joe Molloy, Claude Mallet, André Duesterloh, Igor Bendik, Thomas Hankemeier, James Langridge, Rob J. Vreeken, Giuseppe Astarita
Source:
Analytical Biosciences, LACDR, Leiden University, Leiden, The Netherlands; Netherlands Metabolomics Centre, Leiden University,
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
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Abstract / Summary

In this collaborative work developed by Leiden University, the Netherlands Metabolomics Center, DSM Nutritional Products Europe, and Waters, we present a high-throughput approach to screen and quantify bioactive oxylipins (oxidized fatty acids) in plasma. The combination of solid-phase extraction and UPLC/ESI-tandem quadrupole mass spectrometry provides a comprehensive analysis of oxylipins in a dedicated analytical workflow. Retention times and transitions of 107 oxylipins (including prostaglandins, prostacyclines, thromboxanes, dihydroprostaglandins, and isoprostanes) were annotated for routine high-throughput profiling of plasma samples.

Considering the prominent roles played by oxylipins in health and disease (e.g., inflammation), such a UPLC-based assay could become important as a diagnostic or prognostic screen in nutritional research, clinical research, and drug discovery and development.


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