Targeted Lipidomics of Oxylipins (Oxygenated Fatty Acids)

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Katrin Strassburg, Billy Joe Molloy, Claude Mallet, André Duesterloh, Igor Bendik, Thomas Hankemeier, James Langridge, Rob J. Vreeken, Giuseppe Astarita
Analytical Biosciences, LACDR, Leiden University, Leiden, The Netherlands; Netherlands Metabolomics Centre, Leiden University,
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Biomarker Identification
Biomarker Identification

In this collaborative work developed by Leiden University, the Netherlands Metabolomics Center, DSM Nutritional Products Europe, and Waters, we present a high-throughput approach to screen and quantify bioactive oxylipins (oxidized fatty acids) in plasma. The combination of solid-phase extraction and UPLC/ESI-tandem quadrupole mass spectrometry provides a comprehensive analysis of oxylipins in a dedicated analytical workflow. Retention times and transitions of 107 oxylipins (including prostaglandins, prostacyclines, thromboxanes, dihydroprostaglandins, and isoprostanes) were annotated for routine high-throughput profiling of plasma samples.

Considering the prominent roles played by oxylipins in health and disease (e.g., inflammation), such a UPLC-based assay could become important as a diagnostic or prognostic screen in nutritional research, clinical research, and drug discovery and development.

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