Peptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases

Library Number:
APNT134721106
Part Number:
720004571en
Author(s):
Matthew A. Lauber, Stephan M. Koza, Kenneth J. Fountain
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
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Peptide mapping with FA instead of TFA facilitates more sensitive detection using ESI-MS. As a result, the CSH 180 C18 column is ideal for LC/MS-based peptide mapping because its performance is excellent with either acid modifier. Greater peak capacity and unique selectivity are added benefits of the CSH column.


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