A Generic 2D-UPLC/MS Assay for the Identification and Quantification of Host Cell Proteins in Biopharmaceuticals
This work demonstrates:
- An efficient and generic 2D UPLC/MSE assay is developed for identification and quantification of host cell proteins (HCPs) in biotherapeutics that provides over four to five orders of magnitude in concentration.
- This targeted, high-throughput quantitative assay for selected HCP proteins enables researchers to monitor their abundance changes.
- Additionally, a high-throughput UPLC/MRM-MS assay for monitoring targeted HCPs using tandem quadrupole mass spectrometry can be readily implemented.
***nanoACQUITY UPLC applications readily transfer to the ACQUITY UPLC M-Class System***